Journal: Nuclear Medicine Communications

Article Title: Preclinical evaluation of an antibody-based companion diagnostic for CD44v6 expressing cancer

doi: 10.1097/MNM.0000000000002100

Figure Lengend Snippet: Normalized in vitro specificity, cellular processing, and retention of radiolabeled UU-40 LALA/IAHA on cancer cell lines. (a) Cells were treated with 125 I-, 177 Lu-, or 68 Ga-UU-40 LALA/IAHA either alone (nonblocked) or in the presence of 100-fold molar excess of unlabeled UU-40 LALA/IAHA (blocked). (b) ACT-1 cells were exposed to 10 nM radiolabeled UU-40 LALA/IAHA and evaluated through acid wash for the membrane-bound fraction, and internalized fraction, through harvesting, at 1, 2, 4, 6, and 24 h for 125 I-UU-40 LALA/IAHA and 177 Lu-UU-40 LALA/IAHA . (c) A431 cells were exposed to 10 nM radiolabeled UU-40 LALA/IAHA and evaluated through acid wash for the membrane-bound fraction, and internalized fraction, through harvesting, at 1, 2, 4, 6, and 24 h for 125 I-UU-40 LALA/IAHA and 177 Lu-UU-40 LALA/IAHA . N = 3; error bars represent SD. Student’s t -test was used to determine significance between blocked and nonblocked cells, where P < 0.05 (*).

Article Snippet: The squamous cell carcinoma cell line A431 and the human breast cancer cell line MDA-MB-231 were purchased from the American Type Culture Collection (cat. CRL 1555, RRID:CVCL 0037 and cat. HTB-26, RRID:CVCL_0062, respectively) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) with 10% FBS, 2 mM l -glutamine and antibiotics (100 IU penicillin and 100 μg/mL streptomycin).

Techniques: In Vitro, Membrane

Journal: Nuclear Medicine Communications

Article Title: Preclinical evaluation of an antibody-based companion diagnostic for CD44v6 expressing cancer

doi: 10.1097/MNM.0000000000002100

Figure Lengend Snippet: LigandTracer analyses of radiolabeled UU-40 on A431 cells. (a) LigandTracer curves of 125 I-UU-40 LALA/IAHA or (b) 177 Lu-labeled UU-40 LALA/IAHA on A431 cells. Corresponding InteractionMap analysis of (c) 125 I-UU-40 LALA/IAHA and (d) 177 Lu-UU-40 LALA/IAHA . N ≥ 3.

Article Snippet: The squamous cell carcinoma cell line A431 and the human breast cancer cell line MDA-MB-231 were purchased from the American Type Culture Collection (cat. CRL 1555, RRID:CVCL 0037 and cat. HTB-26, RRID:CVCL_0062, respectively) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) with 10% FBS, 2 mM l -glutamine and antibiotics (100 IU penicillin and 100 μg/mL streptomycin).

Techniques: Labeling

Journal: Nuclear Medicine Communications

Article Title: Preclinical evaluation of an antibody-based companion diagnostic for CD44v6 expressing cancer

doi: 10.1097/MNM.0000000000002100

Figure Lengend Snippet: Biodistribution of 125 I/ 177 Lu-labeled UU-40 LALA/IAHA at 6, 24, and 48 h in ACT-1 xenografts ( n = 4, N = 12) and at 24 and 48 h in A431 xenografts ( n = 2, N = 4). Error bars represent SD.

Article Snippet: The squamous cell carcinoma cell line A431 and the human breast cancer cell line MDA-MB-231 were purchased from the American Type Culture Collection (cat. CRL 1555, RRID:CVCL 0037 and cat. HTB-26, RRID:CVCL_0062, respectively) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) with 10% FBS, 2 mM l -glutamine and antibiotics (100 IU penicillin and 100 μg/mL streptomycin).

Techniques: Labeling

Journal: Nuclear Medicine Communications

Article Title: Preclinical evaluation of an antibody-based companion diagnostic for CD44v6 expressing cancer

doi: 10.1097/MNM.0000000000002100

Figure Lengend Snippet: Small-animal PET/CT and SPECT/CT of a mouse carrying dual A431 xenografts. The animal was injected with dual-nuclide solution containing 68 Ga (3.5 MBq) and 125 I-UU-40 LALA/IAHA (9.1 MBq). (a) PET-imaging at 4 h using 68 Ga-UU-40 LALA/IAHA . (b) SPECT-imaging at 5 h p.i. of 125 I-UU-40 LALA/IAHA . (c) SPECT-imaging at 24 h p.i. of 125 I-UU-40 LALA/IAHA . The same mouse was imaged at all time points ( N = 1).

Article Snippet: The squamous cell carcinoma cell line A431 and the human breast cancer cell line MDA-MB-231 were purchased from the American Type Culture Collection (cat. CRL 1555, RRID:CVCL 0037 and cat. HTB-26, RRID:CVCL_0062, respectively) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) with 10% FBS, 2 mM l -glutamine and antibiotics (100 IU penicillin and 100 μg/mL streptomycin).

Techniques: Positron Emission Tomography-Computed Tomography, Single Photon Emission Computed Tomography, Injection, Imaging

Journal: Cell Death & Disease

Article Title: Gas plasma-induced bacterial PAMP release promotes skin cancer cell death

doi: 10.1038/s41419-025-08283-8

Figure Lengend Snippet: a representative image of gas plasma treatment; b metabolic activity of A375, SCC-25, and A431 cells 24 h post gas plasma treatment and calculated IC 25 values; c metabolic activity of A375, SCC-25, and A431 cells 24 h, 48 h, and 72 h after treatment with three different dilutions of bacterial pPAMP-containing supernatants, statistical analysis was performed using two-way analysis of variance (ANOVA); d metabolic activity of A375, SCC-25 and A431 cells 24 h after gas plasma or PAMP treatment alone or combination treatment, statistical analysis was performed using one-way ANOVA; e representative flow cytometry contour plots of the viable cell population; f quantification of viable cells 24 h after gas plasma or PAMP treatment alone or combination treatment, statistical analysis was performed using two-way ANOVA; g representative flow cytometry histogram of DAPI intensity for cell cycle analysis; h G2/G1 phase ratio in A375, SCC-25 and A431 cells 24 h after gas plasma or PAMP treatment alone or combination treatment, statistical analysis was performed using one-way ANOVA. cPAMPs = PAMPs derived from untreated bacteria. ctrl = control. hPAMPs = PAMPs derived from heat-inactivated bacteria. LPS = lipopolysaccharide. PL = gas plasma. pPAMPs = PAMPs derived from gas plasma-exposed bacteria.

Article Snippet: The human melanoma cell line A375 and the human squamous cell carcinoma cell lines A431, as well as SCC-25 (all ATCC, USA), were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Pan Biotech, Germany) supplemented with 10% fetal bovine serum, 1% penicillin/ streptomycin, and 1% L-glutamine (all Corning, Germany).

Techniques: Clinical Proteomics, Activity Assay, Flow Cytometry, Cell Cycle Assay, Derivative Assay, Bacteria, Control

Journal: Cell Death & Disease

Article Title: Gas plasma-induced bacterial PAMP release promotes skin cancer cell death

doi: 10.1038/s41419-025-08283-8

Figure Lengend Snippet: a representative flow cytometry intensity histograms of CD40 levels in control and treated SCC-25 cells; b heatmap showing medians of calculated expression changes of eight different surface markers in response to gas plasma or PAMP treatment alone or a combination treatment of both ( n = 4); c quantification of intracellular γH2AX expression in A375, SCC-25 and A431 cells 24 h after gas plasma or PAMP treatment alone or combination treatment, violin plots show median (indicated as stacked line) and quartiles (indicated as dotted line), statistical analysis was performed using one-way ANOVA ( n = 4); d representative images of actin cytoskeleton and nucleus staining in control and treated tumor cells; e representative images of DiD, caspase 3/7 and DAPI staining in control and treated A375 cells; f–h fluorescence microscopy-based quantification of the cell number per well 24 h after gas plasma or PAMP treatment alone or combination treatment ( f ) ( n = 4), DAPI intensity per well ( g ) ( n = 4), and caspase 3/7 intensity per well ( h ) ( n = 4) 24 h after gas plasma or PAMP treatment alone or combination treatment, data is shown as box and whiskers (Tukey; mean is shown as ′+′), statistical analysis was performed using unpaired t test (one-tailed). cas 3/7 = caspase 3/7. cPAMPs = PAMPs derived from untreated bacteria. ctrl = control. hPAMPs = PAMPs derived from heat-inactivated bacteria. LPS = lipopolysaccharide. PL = gas plasma. pPAMPs = PAMPs derived from gas plasma-exposed bacteria.

Article Snippet: The human melanoma cell line A375 and the human squamous cell carcinoma cell lines A431, as well as SCC-25 (all ATCC, USA), were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Pan Biotech, Germany) supplemented with 10% fetal bovine serum, 1% penicillin/ streptomycin, and 1% L-glutamine (all Corning, Germany).

Techniques: Flow Cytometry, Control, Expressing, Clinical Proteomics, Staining, Fluorescence, Microscopy, One-tailed Test, Derivative Assay, Bacteria